Antioxidant and Antibacterial Activities of Crude Extracts of Carissa spinarum L. Leave

Abstract

This study's goal was to assess the antioxidant potential of Carissa spinarum leaf extracts in methanol and ethyl acetate. Petroleum ether, chloroform, acetone, ethyl acetate, and methanol were among the solvents used to extract the Carissa spinarum leaf powder. Biochemical tests, specifically diphenylpicrylhydrazyl (DPPH) and hydrogen peroxide radical scavenging activity, were used to evaluate the extracts' antioxidant capacity. The findings showed that the majority of extracts had antibacterial activity and all extracts demonstrated antioxidant activity. Peroxide values ranged from 19.8 meq/kg (90% methanol) to 379 meq/kg (defatted with petroleum ether) at 70 oC, and from 3.1 meq/kg (90% methanol) to 119 meq/kg (defatted with petroleum ether) at room temperature. Ferric reducing antioxidant power was found to range between 336.58 ± 0.052 mgAAE/100 g extract (90% methanol) and 172.94 ± 0.032 mgAAE/100 g (petroleum ether).  With a mean zone of inhibition ranging from 0 to 26 mm, crude extracts of Carissa spinarum leaves demonstrated varying degrees of antibacterial activity against a few common and drug-resistant microbial infections. The crude extract of Carissa spinarum leaves was found to have the strongest bactericidal activities on specific bacterial populations in 90% methanol and pure methanol solvent extracts, while having a weak antibacterial effect on S. aureus, E. coli, S. aurens, and K. pneumoniae in both petroleum ether and chloroform extracts.